Journal article
Measuring fetal brain and lung transcripts in amniotic fluid supernatant: a comparison of digital PCR and RT-qPCR methods
L Hui, S Beard, NJ Hannan
Journal of Maternal Fetal and Neonatal Medicine | TAYLOR & FRANCIS LTD | Published : 2018
Abstract
Purpose: Amniotic fluid (AF) cell-free RNA is a promising source of information regarding fetal physiology. Digital PCR (dPCR) is a direct approach to nucleic acid detection that reports absolute transcript copy number. The aim of this study was to compare quantification of cell-free fetal brain and lung RNA transcripts in AF by reverse transcription-qPCR (RT-qPCR) and dPCR. Material and methods: Prospective hospital-based study was performed in 2016–2017. Pulmonary genes were quantified in term AF samples collected at elective cesarean birth; neurodevelopmental genes were measured in preterm samples (<34 weeks) obtained from women undergoing clinically-indicated amniocentesis. Results: All ..
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Awarded by Sylvia and Charles Viertel Charitable Foundation
Funding Acknowledgements
Funding for this study was provided by the Norman Beischer Medical Research Foundation, the Sylvia Charles Viertel Charitable Foundation, and the Academic Research and Development Committee of Mercy Health Services. The National Health and Medical Research Council provided salary support to L. H. (# 1105603). N. J. H. is supported by a University of Melbourne CR Roper Fellowship. None of the funders had any involvement in the study design, data collection, data analysis, manuscript preparation or publication decision.